Rapid identification of microorganisms from positive blood cultures by MALDI-TOF mass spectrometry subsequent to very short-term incubation on solid medium
نویسندگان
چکیده
منابع مشابه
Rapid identification of mycobacteria from positive MGIT broths of primary cultures by MALDI-TOF mass spectrometry
BACKGROUND Rapid identification of mycobacteria is important for timely treatment and the implementation of public health measures. The MGIT system ensures rapid detection of mycobacteria, but identification is usually delayed by days to weeks due to further subculture on solid medium. Matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF MS) was demonstrated to effective...
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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged as a reliable tool for fast identification and classification of microorganisms. In this regard, it represents a strong challenge to microscopic and molecular biology methods. Nowadays, commercial MALDI systems are accessible for biological research work as well as for diagnostic applications...
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BACKGROUND Faster identification of bacterial isolates from blood cultures can enable earlier clinical intervention for patients with sepsis. We evaluated the clinical impact of direct identification of micro-organisms from positive blood cultures using MALDI-ToF. METHOD Positive blood cultures with organisms seen on Gram stain were included over a four week period. For each patient case, com...
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Leptospirosis is a worldwide deadly zoonotic disease. Accurate identification of the causative Leptospira spp. spirochetes ascertains the pathogenic status of the isolates, identifies potential source of infection and recognises outbreaks. Species identification is currently based on technically demanding, time and resources consuming serological and molecular methods. Matrix-assisted laser des...
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24 A method for rapid species identification of ticks may help clinicians predict the disease outcomes 25 of patients with tick bites and may inform the decision as to whether to administer post-exposure 26 prophylactic antibiotic treatment. We aimed to establish a MALDI-TOF MS spectra database based 27 on the analysis of the legs of 6 tick vectors: Amblyomma variegatum, Rhipicephalus sanguineu...
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ژورنال
عنوان ژورنال: Clinical Microbiology and Infection
سال: 2014
ISSN: 1198-743X
DOI: 10.1111/1469-0691.12640